Isolation and identification of restriction endonuclease BsiKI
نویسندگان
چکیده
منابع مشابه
Isolation and identification of restriction endonuclease BsiSI.
BseBI, an isoschizomer of BstNI (1) has been purified from Bacillus stearothermopilus species. BseBI recognises the sequence 5'...CCWGG...3' (W=A or T) and cleaves between C and W. The enzyme was purified using the following Chromatographic steps: 1. Blue -Sepharose F3GA, 2. Heparin -Sepharose, 3. DEAE-Sepharose. The enzyme was free of contaminating nuclease activity. After 5-fold overdigestion...
متن کاملIsolation and identification of restriction endonuclease SseAI.
SseBI, an isoschizomer of StuI (1) has been purified from Streptomyces species. SseBI recognises the sequence 5' ... AGGCCT ... 3' and cleaves between G and C. The enzyme was purified using the following chromatographic steps: 1. Blue Sepharose F3GA, 2. Heparin-Sepharose. The enzyme was free of contaminating nuclease activity. After 100 fold overdigestion on lambda DNA greater than 95% of the D...
متن کاملIsolation and identification of the restriction endonuclease PtaI from Phormidium tadzschicicum, an isoschizomer of BspMII.
Ptal is a type II restriction endonuclease from the Cyanobacterial strain Phormidium tadzschicicum. This strain was obtained from the Collection of Algal Cultures of Leningrad University. Ptal recognizes the sequence 5'-TCCGGA-3' and cleaves between T and C. The enzyme was purified using the following chromatographic steps: 1) Phosphocelulose, 2) DEAESephadex. The enzyme was free of contaminati...
متن کاملIsolation and identification of restriction endonuclease Asp35HI from Acidiphilium species 35H.
Asp35Hl, a type II restriction endonuclease, has been isolated from Acidiphilium species 35H. As/?35HI, an isoschizomer of Bsml (1), recognizes the six base non-palindromic sequence 5'GAATGC3', and cleaves one nucleotide outside of the recognition sequence on one strand, and within the recognition sequence on the opposite strand, to generate a two base 3' overhang. The enzyme was purified using...
متن کاملChimeric restriction endonuclease.
Fok I restriction endonuclease recognizes the nonpalindromic pentadeoxyribonucleotide 5'-GGATG-3'.5'-CATCC-3' in duplex DNA and cleaves 9 and 13 nt away from the recognition site. Recently, we reported the presence of two distinct and separable domains within this enzyme: one for the sequence-specific recognition of DNA (the DNA-binding domain) and the other for the endonuclease activity (the c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.6.1655